Bioland dNTP Mix 50x 10mM Each – 10×500µL Laboratory Natural Protein

Description

Product Overview

The Bioland dNTP Mix is a high-performance reagent designed for molecular biology applications that require a reliable source of deoxynucleotide triphosphates. Formulated at a 50-fold concentration, each of the four dNTPs (dATP, dCTP, dGTP, and dTTP) is provided at 10 mM, ensuring ample supply for demanding protocols such as polymerase chain reaction (PCR), DNA sequencing, and cloning. The product is supplied in a convenient bulk format of ten 500 µL tubes, allowing laboratories to maintain a ready stock while minimizing waste and handling time. With a package dimension of 12 × 10 × 10 inches and a weight of 25 lb, the mix is shipped in a sturdy container that protects the integrity of the reagents during transport. Since its introduction in December 2014, the Bioland dNTP Mix has earned a solid reputation among researchers for its consistency and stability, reflected in its ranking as #56 in the Lab Natural Proteins subcategory on major e‑commerce platforms.

Each batch undergoes rigorous quality control, including high‑performance liquid chromatography (HPLC) verification and spectrophotometric analysis, to confirm that the concentration of each nucleotide meets the declared 10 mM specification with less than 2 % variance. The reagent is also tested for the absence of contaminating nucleases, ensuring that downstream DNA templates remain intact. Packaging includes a tamper‑evident seal and a desiccant packet to guard against moisture ingress, which can degrade nucleotide quality over time. The product’s long‑term stability at –20 °C for up to twelve months makes it a dependable choice for both short‑term experiments and extended research projects.

Bioland’s commitment to sustainability is reflected in the recyclable packaging materials and the use of environmentally responsible manufacturing processes. The company adheres to ISO‑9001 and ISO‑13485 standards, providing traceability from raw material sourcing to final product release. Customers benefit from a detailed Certificate of Analysis (CoA) that accompanies each shipment, offering transparency about purity, concentration, and sterility metrics. This level of documentation supports regulatory compliance for labs operating under Good Laboratory Practice (GLP) or Good Manufacturing Practice (GMP) guidelines.

Usage

This dNTP solution is optimized for use in standard PCR master mixes, high-fidelity amplification kits, and next-generation sequencing library preparations. The 10 mM concentration matches the requirements of most commercial enzyme formulations, allowing users to add the exact volume needed without additional dilution steps. The 500 µL aliquots are ideal for routine bench work, enabling quick pipetting and reducing the risk of cross‑contamination. Researchers working in academic, biotech, or clinical laboratories will find the mix suitable for a wide range of applications, from routine gene amplification to complex multiplex assays. Its stability at –20 °C for up to twelve months ensures that the reagent remains functional throughout the duration of long‑term projects.

Typical usage guidelines recommend adding 0.5 µL of the 10 mM dNTP mix per 25 µL PCR reaction to achieve a final concentration of 200 µM each nucleotide, which is optimal for most Taq polymerases. For high‑fidelity enzymes that require lower dNTP concentrations, users can adjust the volume accordingly, benefiting from the mix’s flexibility. The reagent also performs well in isothermal amplification methods such as loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA), where the high concentration helps maintain reaction efficiency. When preparing sequencing libraries, the mix provides a reliable source of nucleotides for end‑repair and A‑tailing steps, contributing to high-quality read output.

Storage guidelines emphasize keeping the tubes sealed tightly and avoiding repeated freeze‑thaw cycles. If a tube is inadvertently thawed, it can be returned to –20 °C promptly; the formulation is robust enough to tolerate occasional temperature fluctuations without loss of activity. For laboratories operating in high‑throughput environments, the bulk packaging reduces the frequency of inventory checks and minimizes the chance of stockouts, thereby improving overall workflow efficiency.

Why Choose Us

Bioland is a trusted supplier of life‑science reagents, and the dNTP Mix benefits from rigorous quality‑control processes that include high‑performance liquid chromatography (HPLC) verification and sterility testing. Each batch is produced under ISO‑9001 certified conditions, guaranteeing that the concentration of each nucleotide meets the declared 10 mM specification with a variance of less than 2 %. The product is free from contaminating nucleases, which can degrade DNA templates and compromise experimental outcomes. In addition, Bioland provides responsive technical support, offering guidance on optimal storage, handling, and troubleshooting for any issues that may arise during use. Customers also receive a detailed Certificate of Analysis (CoA) with every purchase, reinforcing confidence in the reagent’s purity and performance.

Beyond quality, Bioland emphasizes customer service and global accessibility. The company maintains regional distribution centers in North America, Europe, and Asia, ensuring rapid delivery times and localized support. Technical experts are available via phone, email, or live chat to assist with protocol optimization, troubleshooting, and product selection. For institutions with strict compliance requirements, Bioland can provide documentation that aligns with GLP, GMP, and ISO‑13485 standards, facilitating audit readiness and regulatory submissions.

Environmental responsibility is another pillar of Bioland’s philosophy. The packaging materials are recyclable, and the manufacturing process incorporates waste‑reduction strategies and energy‑efficient equipment. By choosing Bioland, laboratories not only gain a high‑quality reagent but also support a supplier committed to sustainable practices and continuous improvement.

Key Features

• High‑concentration 10 mM each dNTP reduces the need for frequent re‑preparation.
• Bulk 10 × 500 µL packaging saves storage space and streamlines workflow.
• Validated purity and stability ensure reliable results across diverse protocols.
• ISO‑9001 manufacturing guarantees consistent quality and traceability.
• Dedicated technical support and comprehensive CoA for peace of mind.

FAQ

What is the recommended storage temperature for the dNTP Mix?

Store the reagent at –20 °C in a sealed container. Avoid repeated freeze‑thaw cycles to maintain nucleotide integrity.

Can the dNTP Mix be used with high‑fidelity polymerases?

Yes, the 10 mM concentration is compatible with most high‑fidelity enzymes and does not interfere with their proofreading activity.

How long does the product remain stable after opening?

When kept at –20 °C, the mix retains full activity for at least twelve months after the first opening, provided the tubes are resealed properly.

Is the dNTP Mix free of RNase and DNase contaminants?

Bioland’s production process includes stringent nuclease‑free protocols, ensuring the reagent is free from RNase and DNase activity.

What should I do if I notice precipitation in the solution?

Gently warm the tube to room temperature and vortex briefly. If precipitation persists, replace the affected aliquot with a fresh one from the same batch.