HiMedia M049S-500G Violet Red Bile Agar 500g Laboratory Culture Media

Description

Product Overview

HiMedia M049S-500G Violet Red Bile Agar is a premium dehydrated agar formulation specifically engineered for the selective isolation and enumeration of Gram‑negative enteric bacteria. The product is supplied in a convenient 500 g bulk package, providing ample material for high‑throughput laboratory workflows. Each gram of agar contains precisely calibrated concentrations of bile salts, crystal violet, and other selective agents that inhibit the growth of Gram‑positive organisms while allowing target pathogens to thrive. The medium is prepared by adding distilled water and autoclaving according to standard microbiological protocols, resulting in a clear, nutrient‑rich base that supports robust bacterial growth. The agar’s pH is carefully adjusted to 7.4 ± 0.2, ensuring optimal conditions for the metabolic activities of a wide range of enteric species. The product complies with international quality standards and is manufactured under strict aseptic conditions to guarantee sterility and consistency across batches. Packaging dimensions of 7.36 × 3.31 × 3.03 inches and a net weight of 1.36 lb make storage and handling straightforward for both small research labs and large industrial facilities. HiMedia’s reputation for reliable microbiological media is reflected in the product’s high customer rating and its ranking as a top seller in the Lab Natural Proteins category.

Usage

This agar is ideally suited for a variety of microbiological applications, including clinical diagnostics, food safety testing, environmental monitoring, and pharmaceutical quality control. In clinical laboratories, it is frequently employed to isolate Salmonella, Shigella, and other pathogenic Enterobacteriaceae from stool specimens, enabling rapid identification and antimicrobial susceptibility testing. Food industry professionals use the medium to detect contamination in raw meat, dairy products, and processed foods, ensuring compliance with regulatory standards. Environmental scientists apply the agar to water and soil samples to monitor the presence of fecal coliforms, providing valuable data for public health assessments. The product’s versatility extends to academic settings, where it serves as a teaching tool for students learning aseptic techniques, selective media preparation, and bacterial identification. For optimal results, the agar should be reconstituted with 1 L of distilled water, autoclaved at 121 °C for 15 minutes, and poured into sterile Petri dishes while still molten. Plates are allowed to solidify at room temperature, then stored inverted at 2‑8 °C for up to four weeks. When inoculating, a calibrated loop or swab is used to spread the sample evenly across the surface, and plates are incubated aerobically at 35‑37 °C for 24‑48 hours. Colonies of target Gram‑negative organisms typically appear as pink to red, smooth, and circular, facilitating easy visual differentiation from background flora.

Why Choose Us

Choosing HiMedia’s Violet Red Bile Agar means selecting a product backed by decades of expertise in microbiological media development. The formulation is the result of rigorous research and validation studies that confirm its selectivity, sensitivity, and reproducibility across diverse sample types. Quality assurance processes include raw material testing, in‑process monitoring, and final product verification to ensure each batch meets stringent performance criteria. The agar is free from animal‑derived components, making it suitable for laboratories that require non‑animal based media. HiMedia provides comprehensive technical support, including detailed protocols, troubleshooting guides, and direct access to experienced microbiologists who can assist with method optimization. Customers benefit from fast shipping, reliable inventory management, and the option to purchase in bulk for cost‑effective procurement. Additionally, the company offers a satisfaction guarantee and responsive after‑sales service, reinforcing confidence in the product’s long‑term reliability. By integrating HiMedia’s Violet Red Bile Agar into your workflow, you gain a competitive edge through accurate detection, reduced false‑positive rates, and streamlined laboratory operations.

Key Features

• Highly selective formulation suppresses Gram‑positive flora while promoting target Gram‑negative growth.
• Consistent performance with batch‑to‑batch uniformity verified by strict quality control.
• Convenient 500 g bulk size reduces the need for frequent reordering and minimizes waste.
• Easy preparation: dissolve, autoclave, and pour – suitable for both novice and experienced technicians.
• Dedicated technical support and after‑sales service ensure reliable usage and rapid issue resolution.

FAQ

What types of bacteria can be isolated with this agar?

Violet Red Bile Agar is designed to isolate Gram‑negative enteric bacteria such as Salmonella, Shigella, and other members of the Enterobacteriaceae family. It is also effective for detecting fecal coliforms in environmental samples.

How long can the prepared plates be stored?

After solidification, plates can be stored inverted at 2‑8 °C for up to four weeks without loss of selectivity or performance. For best results, use plates within two weeks of preparation.

Is this medium suitable for clinical diagnostic laboratories?

Yes, the agar meets the requirements of clinical microbiology protocols for the isolation of pathogenic Gram‑negative organisms from clinical specimens, and it is widely used in hospital laboratories worldwide.

Can the agar be used for educational purposes?

Absolutely. The clear colony morphology and selective properties make it an excellent teaching tool for microbiology courses, allowing students to observe differential growth patterns and learn aseptic techniques.

What support does HiMedia provide if I encounter issues?

HiMedia offers technical assistance via phone, email, and online resources. Our experts can help troubleshoot preparation problems, interpret results, and optimize protocols for specific applications.