Peptone RPI P20240-100.0 Powder 100g Natural Protein for Lab Research

Description

Product Overview

The RPI P20240-100.0 Peptone powder is a premium-grade, natural protein source designed specifically for laboratory and research applications. Each 100 g package contains a finely milled, low‑dust powder that delivers a total nitrogen (TN) content ranging from 15.0% to 16.0% and an amino nitrogen (AN) content between 3.0% and 3.5%. The AN/TN ratio of 0.21‑0.23 ensures a balanced amino acid profile, supporting robust cell growth and metabolic activity in a variety of biological systems. Manufactured by Research Products International (RPI) in Mexico, the product meets stringent quality standards and is packaged in a compact, resealable container measuring 3.5 × 3.5 × 3.75 inches, weighing just 3.53 ounces. This size and weight make it convenient for storage in standard laboratory freezers and cabinets while providing enough material for multiple experiments. The powder is free from extraneous additives, preservatives, or contaminants, guaranteeing purity and reproducibility across batches. Its consistent particle size distribution facilitates precise weighing and dissolution, reducing variability in experimental protocols.

Usage

Peptone is a versatile supplement used across a broad spectrum of life‑science workflows. In cell culture, it serves as a primary nitrogen source, supporting the growth of mammalian, insect, and microbial cells in both serum‑free and serum‑containing media. Researchers often incorporate Peptone at concentrations ranging from 0.5 g/L to 5 g/L, depending on the specific cell line and desired growth kinetics. In microbiology, the powder provides essential nutrients for the cultivation of bacteria, yeast, and fungi, enhancing colony formation and biomass yield in broth and agar media. Enzyme assay development also benefits from Peptone’s stable nitrogen content, which helps maintain optimal pH and ionic strength during kinetic measurements. The product’s low‑dust formulation minimizes aerosol generation, making it safer to handle in biosafety cabinets and reducing the risk of cross‑contamination. Because the powder dissolves readily in aqueous solutions, it can be autoclaved or filter‑sterilized without loss of functionality, ensuring sterility for sensitive applications such as vaccine production, recombinant protein expression, and metabolic engineering studies.

Why Choose Us

Choosing RPI’s Peptone powder means selecting a product backed by decades of experience in supplying high‑quality biomolecules to the scientific community. Each batch undergoes rigorous testing for nitrogen content, microbial contamination, and endotoxin levels, providing confidence that the material will perform consistently in critical experiments. The manufacturing process adheres to Good Manufacturing Practices (GMP), and the facility is regularly audited to maintain compliance with international standards. RPI’s commitment to traceability ensures that every container can be linked to its production lot, enabling precise documentation for regulatory submissions and reproducibility studies. Additionally, the company offers responsive technical support, with scientists available to answer formulation questions, troubleshoot assay issues, and provide guidance on optimal usage concentrations. Customers benefit from a reliable supply chain, with inventory management options that include bulk purchasing and scheduled deliveries to keep laboratories stocked without interruption. The combination of product purity, documented performance, and dedicated service makes RPI Peptone a trusted choice for academic, industrial, and biotech research environments.

Key Features

• High total nitrogen (15‑16%) delivers robust protein content for superior cell growth and productivity.
• Precise amino nitrogen ratio (0.21‑0.23) ensures balanced amino acid availability across diverse biological systems.
• Low‑dust, fine‑grade powder enables accurate weighing, easy dissolution, and safe handling in biosafety cabinets.
• Manufactured in Mexico under GMP conditions, providing consistent quality and full batch traceability.
• Dedicated technical support and flexible supply options help streamline research workflows and reduce downtime.

FAQ

What is the recommended concentration of Peptone for mammalian cell culture?

For most mammalian cell lines, a starting concentration of 1 g/L to 2 g/L is typical. The exact amount may be adjusted based on cell line sensitivity, serum presence, and desired growth rate. It is advisable to perform a small‑scale titration experiment to identify the optimal concentration for your specific application.

Can the Peptone powder be sterilized by autoclaving?

Yes, the powder can be autoclaved at 121 °C for 15 minutes without degradation of its nitrogen content. Autoclaving is a common practice to ensure sterility for cell culture media. Alternatively, the solution can be filter‑sterilized using a 0.22 µm membrane if heat‑sensitive components are present in the formulation.

Is the product free from endotoxins and other microbial contaminants?

RPI conducts endotoxin testing on each batch, and the results consistently fall well below the limits set by the United States Pharmacopeia (USP) for laboratory reagents. The powder is also screened for bacterial and fungal contamination, guaranteeing a clean product suitable for sensitive downstream applications such as recombinant protein production.

How should the powder be stored to maintain its stability?

Store the container in a cool, dry place away from direct sunlight. For long‑term storage, keep the product at 2‑8 °C in a sealed container to prevent moisture absorption. Once opened, reseal the container tightly and use within six months to ensure optimal performance.

What support does RPI provide if I encounter issues with the product?

RPI offers a dedicated technical support team reachable by phone or email. Customers can receive formulation advice, troubleshooting assistance, and detailed product documentation. In addition, RPI can provide sample batches for method development and validation, helping you integrate the Peptone powder smoothly into your research protocols.