2×YT Medium Powder CryoKing Culture Media 250g Bottle High‑Nutrient

Description

Product Overview

The 2×YT Medium is a premium, high‑nutrient powder designed specifically for the cultivation of Escherichia coli in research laboratories. Formulated by CryoKing, the medium delivers a precise balance of tryptone (16 g per litre), yeast extract (10 g per litre) and sodium chloride (5 g per litre), creating an environment rich in carbon, nitrogen, vitamins, and essential minerals. This composition supports rapid cell division while maintaining the physiological characteristics required for downstream molecular biology applications such as plasmid isolation, protein expression, and genetic manipulation. The final pH of the medium is adjusted to 7.2 ± 0.2 at 25 °C, ensuring consistency across batches and reproducible experimental outcomes. Packaged in a 250 g dry‑powder bottle, the product is stable, easy to store, and resistant to moisture ingress, making it suitable for long‑term inventory management in busy laboratory settings.

Usage

The 2×YT Medium is intended for use in standard microbiology and molecular biology protocols that require robust growth of E. coli. To prepare, dissolve 31 g of the powder in one litre of purified water, stir until fully dissolved, and then autoclave at 121 °C for 20 minutes to ensure sterility. After cooling, the medium can be inoculated with a starter culture or directly with a glycerol stock, providing a reliable platform for routine cloning, transformation, and expression experiments. Its high nutrient content makes it especially valuable for applications that demand high cell density, such as large‑scale protein production or plasmid yield optimization. The medium is compatible with a wide range of laboratory equipment, including shake flasks, bioreactors, and microtiter plates, allowing flexibility in experimental design. Researchers working in academic, industrial, or biotech environments will find the 2×YT Medium suitable for both small‑scale pilot studies and larger production runs.

Why Choose Us

CryoKing has a long history of supplying high‑quality life‑science reagents, and the 2×YT Medium reflects the company’s commitment to consistency, performance, and customer support. Each batch undergoes rigorous quality control testing, including verification of component concentrations, pH stability, and sterility assurance, guaranteeing that users receive a product that meets exacting scientific standards. The medium’s formulation has been optimized to reduce variability caused by fluctuations in raw material quality, delivering reproducible results even in demanding experimental workflows. In addition, CryoKing provides detailed technical datasheets, preparation guidelines, and troubleshooting resources to help users achieve the best possible outcomes. Should any issues arise, the dedicated technical support team is available to assist with protocol optimization, troubleshooting, and product inquiries, ensuring that laboratories can maintain productivity without interruption. By choosing CryoKing’s 2×YT Medium, scientists gain a reliable, cost‑effective solution that supports the full spectrum of bacterial culture needs.

Key Features

• High‑nutrient composition delivers rapid and dense E. coli growth, reducing incubation time.
• Precise pH buffering ensures batch‑to‑batch consistency for reproducible experiments.
• Easy preparation: dissolve, autoclave, and use – no additional supplements required.
• Stable 250 g powder packaging minimizes waste and simplifies inventory management.
• Responsive technical support provides guidance and troubleshooting for optimal results.

FAQ

What is the optimal incubation temperature for cultures grown in 2×YT Medium?

The medium supports typical E. coli growth at 37 °C, which is the standard temperature for most molecular biology applications. However, the formulation is also suitable for lower temperatures (30‑25 °C) when slower growth or specific protein folding conditions are required. Adjusting temperature can be used to fine‑tune expression levels or to accommodate temperature‑sensitive strains.

Can the 2×YT Medium be used for organisms other than E. coli?

While the medium is specifically optimized for E. coli, its rich nutrient profile can support the growth of other Gram‑negative bacteria such as Salmonella or Pseudomonas species. For organisms with different nutritional requirements, users may need to supplement the medium with additional carbon or nitrogen sources, or adjust the salt concentration to match the target organism’s physiology.

Is it necessary to add additional vitamins or minerals to the medium?

The 2×YT formulation already contains a comprehensive mix of vitamins and minerals supplied by the yeast extract component, which typically satisfies the needs of standard laboratory strains. For auxotrophic mutants that require specific supplements, users can add the required nutrients after autoclaving to avoid degradation, following the manufacturer’s recommended concentrations.

How long can the prepared medium be stored before use?

After autoclaving, the sterile 2×YT Medium can be stored at 4 °C for up to two weeks without significant loss of performance. For longer storage, it is advisable to aliquot the medium into sterile containers and keep them frozen at –20 °C, where the medium remains stable for several months. Thawing should be done gently to avoid precipitation of components.

What troubleshooting steps should I take if I observe low cell density?

If cultures exhibit unexpectedly low growth, first verify that the powder was fully dissolved before autoclaving and that the correct amount (31 g per litre) was used. Check the pH of the medium after cooling; deviations outside the 7.0‑7.4 range can affect growth. Ensure that the inoculum is healthy and that incubation conditions (temperature, shaking speed) are optimal. If the problem persists, contact CryoKing technical support for detailed assistance.