Zymo Research Zymo-Spin IIC Column 250 – 25 µg DNA/50 µg RNA, 900 µl

Description

Product Overview

The Zymo-Spin IIC Column is engineered for laboratories that demand reliable, high‑throughput purification of nucleic acids and natural proteins. Each column in the 250‑pack offers a binding capacity of 25 µg DNA and 50 µg RNA, allowing researchers to process multiple samples without compromising yield. The column features a volumetric capacity of 900 µl, providing ample space for sample loading and washing steps, while the elution volume of at least 25 µl ensures concentrated recovery of target molecules. Constructed from chemically inert materials, the resin maintains stability across a broad pH range and resists degradation during repeated use. The compact design fits standard 2 ml microcentrifuge tubes, facilitating seamless integration into existing workflows. Package dimensions of 12 × 12 × 12 inches and a weight of 12 pounds make storage and handling straightforward for busy laboratory environments.

Usage

Designed for versatility, the Zymo-Spin IIC Column excels in a variety of applications, including plasmid DNA purification, RNA isolation, and protein‑nucleic acid complex extraction. Its high binding capacity is particularly advantageous for protocols that require the recovery of low‑abundance targets from complex lysates. The column performs optimally in both manual spin‑column formats and automated liquid‑handling systems, making it suitable for small‑scale research projects as well as larger, high‑throughput screening campaigns. Users in academic, biotech, and pharmaceutical settings can rely on the column’s consistent performance to reduce variability and improve reproducibility across experiments. The robust construction also tolerates harsh washing buffers, enabling stringent removal of contaminants while preserving the integrity of the bound biomolecules.

Why Choose Us

Zymo Research brings decades of expertise in nucleic acid purification technology, and the IIC Column reflects that legacy of quality and innovation. Each column undergoes rigorous quality control testing to verify binding capacity, flow consistency, and contaminant‑free performance. The 250‑pack configuration provides cost‑effective scalability, reducing per‑sample expense without sacrificing reliability. Our dedicated technical support team offers guidance on protocol optimization, troubleshooting, and method development, ensuring that users achieve the best possible outcomes. Additionally, Zymo Research adheres to strict manufacturing standards, guaranteeing batch‑to‑batch consistency and compliance with industry regulations. Choosing the Zymo-Spin IIC Column means investing in a proven solution backed by a reputable brand committed to scientific excellence.

Key Features

• High binding capacity of 25 µg DNA and 50 µg RNA per column for efficient recovery.
• Large volumetric capacity of 900 µl accommodates diverse sample volumes and washing steps.
• Elution volume of ≥25 µl provides concentrated product suitable for downstream applications.
• Chemically inert resin ensures stability across a wide pH range and resistance to degradation.
• Comprehensive technical support and detailed protocols from Zymo Research enhance user success.

FAQ

What sample types are compatible with the Zymo-Spin IIC Column?

The column is compatible with a broad range of biological samples, including plasmid DNA, genomic DNA, total RNA, mRNA, and protein‑nucleic acid complexes. It works well with lysates prepared in common buffers and can handle both purified and crude extracts.

Can the column be used in an automated workflow?

Yes, the Zymo-Spin IIC Column is designed to fit standard 2 ml microcentrifuge tube formats, making it compatible with many automated liquid‑handling platforms. Users can program spin‑column steps into their automation scripts for high‑throughput processing.

How many times can a single column be reused?

Each column is intended for single‑use applications to ensure optimal performance and prevent cross‑contamination. For projects requiring multiple purifications, the 250‑pack provides ample supply for repeated use.

What is the recommended storage condition for the columns?

Store the columns at room temperature, protected from moisture and direct sunlight. The sealed packaging maintains column integrity for the duration of the product’s shelf life.

Is there a protocol available for simultaneous DNA and RNA purification?

Zymo Research provides detailed protocols that allow simultaneous extraction of DNA and RNA from the same sample using the IIC Column. These protocols outline buffer compositions and step‑by‑step instructions to maximize yield and purity for both nucleic acid types.