HiMedia M425-500G Yeast Malt Broth 500g Laboratory Media

Description

Product Overview

The HiMedia M425-500G Yeast Malt Broth is a premium laboratory medium formulated to deliver consistent, high‑performance results for a wide range of microbial culture applications. Each 500 g package contains a finely milled, fully soluble powder that dissolves quickly in distilled water, producing a clear, nutrient‑rich broth ideal for both bacterial and fungal growth. The formulation includes a balanced blend of malt extract, yeast extract, peptones, and essential minerals, providing carbon, nitrogen, vitamins, and trace elements required for robust cell proliferation. Package dimensions measure 7.4 × 3.39 × 3.31 inches (18.8 × 8.6 × 8.4 cm) and the net weight is 0.612 kg, making it easy to store and handle in standard laboratory environments. Manufactured by HiMedia Laboratories in India, this product complies with international quality standards and is listed under the Natural Proteins category, reflecting its suitability for protein‑rich culture media. The broth is sterile‑filtered and ready for autoclave sterilization, ensuring a contaminant‑free starting point for sensitive experiments. Its reliable performance has earned it a ranking among the top lab natural proteins on major e‑commerce platforms, confirming its acceptance by researchers worldwide.

Usage

Designed for versatility, the HiMedia Yeast Malt Broth can be employed in a variety of microbiological and biotechnological workflows. Typical usage includes routine cultivation of non‑fastidious bacteria such as Escherichia coli, Staphylococcus aureus, and Enterococcus faecalis, as well as fungi like Candida albicans and Aspergillus niger. Researchers often use this medium for preliminary growth assessments, antibiotic susceptibility testing, and as a base for more complex selective or differential media formulations. The broth supports both batch and fed‑batch fermentation processes, making it valuable for small‑scale pilot studies and larger production runs. Because the powder dissolves without clumping, it is ideal for automated liquid handling systems and high‑throughput screening platforms. The product’s stability at room temperature allows laboratories to maintain a ready stock, reducing preparation time and minimizing variability between batches. When preparing the medium, dissolve 40 g of powder in 1 L of distilled water, adjust the pH to 7.0 ± 0.2 if required, and sterilize by autoclaving at 121 °C for 15 minutes. After cooling, the broth can be aliquoted into sterile containers for immediate use or stored at 2‑8 °C for up to two weeks without loss of performance.

Why Choose Us

Choosing HiMedia’s Yeast Malt Broth means partnering with a brand that has over three decades of experience delivering high‑quality life‑science reagents. Each batch undergoes rigorous quality control testing, including sterility checks, nutrient composition verification, and performance validation in multiple laboratory settings. The manufacturing facility follows Good Manufacturing Practices (GMP) and is ISO‑9001 certified, ensuring traceability and consistency across production runs. HiMedia provides comprehensive technical support, offering guidance on medium preparation, troubleshooting, and protocol optimization through a dedicated customer service team reachable via phone, email, or online chat. In addition, the company offers a satisfaction guarantee and a clear return policy, reinforcing confidence in the product’s reliability. By sourcing raw materials from reputable suppliers and employing state‑of‑the‑art processing equipment, HiMedia minimizes batch‑to‑batch variation, a critical factor for reproducible scientific results. The product’s packaging is designed for durability, protecting the powder from moisture ingress and physical damage during shipping and storage. Moreover, HiMedia’s commitment to sustainability is reflected in its use of recyclable packaging materials and environmentally responsible manufacturing processes, aligning with the values of modern research institutions.

Key Features

• High‑quality nutrient composition delivers consistent growth for a broad spectrum of microorganisms.
• Finely milled powder ensures rapid, clump‑free dissolution for precise media preparation.
• Stable at room temperature, providing a long shelf‑life and reducing waste in busy labs.
• Comes in a 500 g bulk package, offering cost‑effective supply for routine and large‑scale applications.
• Dedicated technical support and clear return policy guarantee reliable after‑sales service.

FAQ

What types of microorganisms can be cultured in this broth?

The Yeast Malt Broth supports the growth of a wide range of bacteria and fungi, including common laboratory strains such as E. coli, S. aureus, Pseudomonas aeruginosa, as well as yeasts like Candida albicans and filamentous fungi such as Aspergillus niger. Its balanced nutrient profile makes it suitable for both aerobic and facultative anaerobic organisms.

How should the medium be sterilized before use?

After dissolving the recommended amount of powder (typically 40 g per litre of distilled water), the solution should be autoclaved at 121 °C for 15 minutes. This standard sterilization cycle eliminates contaminating spores and ensures a sterile environment for downstream experiments. If a filtration method is preferred, the broth can be passed through a 0.22 µm membrane filter under aseptic conditions.

Can the broth be modified for selective or differential applications?

Yes, the base Yeast Malt Broth can be supplemented with additional components such as antibiotics, dyes, or specific carbon sources to create selective or differential media. Researchers often add glucose, lactose, or specific amino acids to tailor the medium for particular metabolic studies or to isolate resistant strains.

What storage conditions are recommended for the powder?

Store the unopened package in a cool, dry place away from direct sunlight. Once opened, keep the powder in a tightly sealed container to prevent moisture absorption. Under these conditions, the product retains its full potency for at least 12 months.

Is technical assistance available for troubleshooting preparation issues?

HiMedia provides dedicated technical support staffed by experienced scientists. Users can contact the support team via phone, email, or the online portal for assistance with media preparation, pH adjustment, sterilization protocols, or any unexpected growth outcomes. The team also offers downloadable SOPs and troubleshooting guides to help resolve common issues quickly.