HiMedia Bile Esculin Agar Modified 500g Natural Protein Media

Description

Product Overview

HiMedia Laboratories presents the M972A-500G Bile Esculin Agar, a specially formulated modified agar designed for the cultivation and differentiation of bile‑esculin positive microorganisms within the natural protein research domain. This medium combines a balanced blend of peptones, agar, and selective agents that promote robust growth while suppressing unwanted flora, ensuring clear and interpretable results. The 500‑gram bulk packaging offers laboratories a cost‑effective solution for high‑throughput screening, quality control, and routine diagnostic workflows. Each gram of agar is meticulously prepared to meet stringent quality standards, providing consistent pH, moisture content, and sterility across batches. The product’s physical specifications include compact dimensions of 7.5 × 3.5 × 3 inches and a lightweight profile of 1.41 ounces, facilitating easy storage and handling in standard laboratory environments. Manufactured in India under the HiMedia brand, the agar adheres to international regulatory guidelines, making it suitable for both academic and industrial applications.

Usage

This agar is ideal for microbiologists, clinical diagnosticians, and research scientists who require a reliable medium for isolating and identifying Enterococcus species, particularly those that hydrolyze esculin in the presence of bile salts. The medium supports growth at temperatures ranging from 35 °C to 37 °C, with an incubation period of 24‑48 hours yielding distinct blackening of the agar due to esculin hydrolysis. It is frequently employed in quality control protocols for pharmaceutical manufacturing, water testing, and food safety assessments where detection of bile‑esculin positive organisms is critical. Users can prepare plates by pouring the molten agar into sterile Petri dishes, allowing it to solidify, and then inoculating with the test organism using standard streaking techniques. The clear differentiation of colonies—blackening versus non‑blackening—facilitates rapid visual identification, reducing the need for additional biochemical tests. For optimal results, laboratories should follow aseptic techniques, store the agar at 2‑8 °C, and avoid prolonged exposure to moisture or direct sunlight.

Why Choose Us

Choosing HiMedia’s Bile Esculin Agar means partnering with a trusted leader in life‑science reagents that prioritizes product integrity, scientific rigor, and customer support. Each batch undergoes comprehensive quality control, including sterility testing, performance verification, and batch‑to‑batch consistency checks, ensuring that researchers receive a medium that performs exactly as expected. The formulation is optimized for clarity of results, minimizing background interference and enhancing the contrast between positive and negative colonies. HiMedia’s extensive experience in microbiological media development translates into a product that is both user‑friendly and highly reliable, reducing experimental variability and accelerating project timelines. Moreover, the company offers responsive technical assistance, detailed usage guidelines, and a dedicated after‑sales service team ready to address any queries or concerns, reinforcing confidence in the purchase and long‑term usage of the product.

Key Features

• Consistent batch quality ensures reproducible results across experiments.
• Selective formulation promotes growth of bile‑esculin positive organisms while inhibiting contaminants.
• Clear blackening reaction provides rapid visual identification without additional tests.
• 500 g bulk size offers economical supply for high‑volume laboratory workflows.
• Dedicated technical support and comprehensive documentation for seamless integration.

FAQ

What organisms can be identified using this agar?

The Bile Esculin Agar is primarily used to isolate and differentiate Enterococcus species, especially Enterococcus faecalis and Enterococcus faecium, which hydrolyze esculin in the presence of bile salts. It can also aid in detecting other bile‑esculin positive Gram‑positive cocci, providing a valuable tool for clinical and environmental microbiology.

How should the agar be stored to maintain its effectiveness?

For optimal shelf life, store the agar plates at 2 °C to 8 °C in a dry, dark environment. Avoid exposure to moisture, direct sunlight, or temperature fluctuations, as these can affect the agar’s consistency and the activity of selective agents. Properly sealed packaging helps preserve sterility and performance.

Can this medium be used for quantitative assays?

Yes, the agar can support quantitative colony‑forming unit (CFU) assays when combined with appropriate dilution and plating techniques. Its selective nature ensures that only target organisms grow, allowing accurate enumeration of bile‑esculin positive bacteria in mixed samples.

Is the product compatible with automated plate handling systems?

Absolutely. The agar’s standard Petri dish dimensions and consistent thickness make it compatible with most automated inoculation and imaging platforms used in high‑throughput laboratories. Users should verify compatibility with specific equipment manufacturers for optimal performance.

What support does HiMedia provide if I encounter issues?

HiMedia offers a dedicated customer service line, email support, and online resources including detailed protocols, troubleshooting guides, and FAQs. If any quality concerns arise, the company will arrange for product replacement or investigation in accordance with its quality assurance policies.